6H3G

Alcohol oxidase from Phanerochaete chrysosporium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-30
Detector	DECTRIS PILATUS3 X 2M
Wavelength(s)	0.9726
Spacegroup name	P 1 21 1
Unit cell lengths	112.665, 204.037, 116.520
Unit cell angles	90.00, 105.02, 90.00

Refinement procedure

Resolution	49.320 - 2.600
R-factor	0.19618
Rwork	0.193
R-free	0.26394
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hsa
RMSD bond length	0.006
RMSD bond angle	1.215
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.320	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.133	1.092
Number of reflections	151989
<I/σ(I)>	6.9
Completeness [%]	97.8
Redundancy	3.6
CC(1/2)	0.990	0.394

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	14% (w/v) PEG3350 and 0.2 M potassium acetate