6H3G
Alcohol oxidase from Phanerochaete chrysosporium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-30 |
| Detector | DECTRIS PILATUS3 X 2M |
| Wavelength(s) | 0.9726 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 112.665, 204.037, 116.520 |
| Unit cell angles | 90.00, 105.02, 90.00 |
Refinement procedure
| Resolution | 49.320 - 2.600 |
| R-factor | 0.19618 |
| Rwork | 0.193 |
| R-free | 0.26394 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hsa |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.215 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.320 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.133 | 1.092 |
| Number of reflections | 151989 | |
| <I/σ(I)> | 6.9 | |
| Completeness [%] | 97.8 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.990 | 0.394 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 14% (w/v) PEG3350 and 0.2 M potassium acetate |
