6H35
Myxococcus xanthus MglA bound to GDP and Pi with mixed inactive and active switch region conformations
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 88.600, 116.650, 49.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.110 - 2.300 |
| R-factor | 0.2155 |
| Rwork | 0.213 |
| R-free | 0.25660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.764 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.299 | 2.299 |
| Rmerge | 0.160 | |
| Rpim | 0.076 | |
| Number of reflections | 93193 | 474 |
| <I/σ(I)> | 8.29 | 1.03 |
| Completeness [%] | 74.7 | 20.5 |
| Redundancy | 5.3 | |
| CC(1/2) | 0.990 | 0.392 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1 M HEPES pH 7.5, 70% MPD |
