6H31
Staphylopine dehydrogenase in the apo state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97242 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.840, 49.000, 155.140 |
| Unit cell angles | 90.00, 94.59, 90.00 |
Refinement procedure
| Resolution | 19.980 - 2.300 |
| R-factor | 0.19227 |
| Rwork | 0.190 |
| R-free | 0.24411 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gmz |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.915 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 178459 | |
| <I/σ(I)> | 8.7 | |
| Completeness [%] | 98.3 | 99.1 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.15 M Potassium bromide, 30% PEG MME 2000 |
