6H2J

Crystal structure of the HsdR subunit of the EcoR124I restriction enzyme with the C-terminal domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X12
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X12
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-20
Detector	MARRESEARCH
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	123.910, 129.922, 161.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.968 - 2.600
R-factor	0.2035
Rwork	0.203
R-free	0.26380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2w00 5j3n
RMSD bond length	0.009
RMSD bond angle	1.008
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	Auto-Rickshaw
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.640
High resolution limit [Å]	2.600	2.600
Number of reflections	80316
<I/σ(I)>	18.2
Completeness [%]	100.0
Redundancy	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	1 UL OF PROTEIN IN 20 MM PHOSPHATE PH 7.5, 100 MM KCL, 5 MM ATP WAS MIXED WITH 2 UL OF RESERVOIR, CONTAINING 0.2 M LI2SO4, 8 % PEG 20K, 8 % PEG 550 MME, 1.5 MM DTT