6H26

Rabbit muscle phosphoglycerate mutase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-03
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9184
Spacegroup name	P 41 21 2
Unit cell lengths	71.308, 71.308, 112.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.987 - 1.288
R-factor	0.1525
Rwork	0.152
R-free	0.18680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yfk
RMSD bond length	0.010
RMSD bond angle	1.073
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.990	1.370
High resolution limit [Å]	1.288	1.288
Rmerge	0.057	1.155
Number of reflections	73677	11616
<I/σ(I)>	31.05	2.88
Completeness [%]	99.7	98.6
Redundancy	18.82	16.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	292	0.2 M NaCl, 20%PEG6000, 100 mM HEPES