6H21
TarP-UDP-GlcNAc-Mn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-27 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.91840 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.860, 95.360, 130.550 |
| Unit cell angles | 90.00, 93.51, 90.00 |
Refinement procedure
| Resolution | 47.680 - 1.800 |
| R-factor | 0.17744 |
| Rwork | 0.176 |
| R-free | 0.21149 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6h1j |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.277 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.700 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 99074 | |
| <I/σ(I)> | 12 | |
| Completeness [%] | 99.8 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 293 | 25% PEG 3350, 250 mM magnesium chloride, 0.1 M sodium citrate, pH 5.7 |
