6H1Q
Proteus mirabilis Ambient Temperature Fimbriae adhesin AtfE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-03 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.913, 103.793, 67.758 |
| Unit cell angles | 90.00, 95.83, 90.00 |
Refinement procedure
| Resolution | 41.120 - 1.580 |
| R-factor | 0.1799 |
| Rwork | 0.179 |
| R-free | 0.20270 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.955 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.930 | 1.610 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.138 | |
| Rmeas | 0.161 | |
| Number of reflections | 68486 | 3315 |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 98.0 | 95.8 |
| Redundancy | 7.3 | 7.4 |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.8 M NaH2PO4, 0.8 M KH2PO4, 0.1 M Hepes pH 7.5 |
