6H1N

Crystal Structure of a Zebra-fish pro-survival protein NRZ-apo

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 43
Unit cell lengths	48.177, 48.177, 75.332
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.177 - 2.000
R-factor	0.192
Rwork	0.190
R-free	0.22370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fbx
RMSD bond length	0.012
RMSD bond angle	1.269
Data reduction software	xia2
Data scaling software	Aimless (7.0.046)
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.180	8.940
High resolution limit [Å]	2.000	2.000
Rmerge	0.051
Rmeas	0.056
Rpim	0.029
Number of reflections	11653	834
<I/σ(I)>	18.3
Completeness [%]	99.9	99.5
Redundancy	6.9	6.9
CC(1/2)	1.000	0.615

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	1.0 M magnesium sulphate hydrate, 0.1 M sodium acetate trihydrate pH 4.6