6H0T
Crystal structure of native recombinant human bile salt activated lipase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.274, 97.676, 109.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.930 - 1.900 |
| R-factor | 0.2117 |
| Rwork | 0.210 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f6w |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.848 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.930 | 1.968 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.085 | 1.231 |
| Rmeas | 0.096 | 1.386 |
| Rpim | 0.044 | 0.622 |
| Number of reflections | 49920 | 4887 |
| <I/σ(I)> | 8.07 | |
| Completeness [%] | 99.5 | 99.17 |
| Redundancy | 4.5 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 8000, cacodylate, zinc acetate |
