6H0N
The structure of wild-type Arabidopsis thaliana UDP-apiose/UDP-xylose synthase in complex with NAD+ and UDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-27 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.003680 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 146.028, 146.028, 132.845 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 126.460 - 3.020 |
| R-factor | 0.20944 |
| Rwork | 0.207 |
| R-free | 0.25278 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3slg |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.824 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 126.460 | 3.180 |
| High resolution limit [Å] | 3.020 | 3.020 |
| Rmerge | 0.146 | 0.800 |
| Number of reflections | 32264 | 4488 |
| <I/σ(I)> | 9.2 | 1 |
| Completeness [%] | 98.9 | 95.4 |
| Redundancy | 4.4 | 4.5 |
| CC(1/2) | 0.992 | 0.378 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.5 | 293 | 0.8 M sodium phosphate, 0.8 M potassium phosphate, 0.1 M sodium-HEPES |
