6H0K

Hen egg-white lysozyme structure determined with data from the EuXFEL, the first MHz free electron laser, 7.47 keV photon energy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	EUROPEAN XFEL BEAMLINE SPB/SFX
Synchrotron site	European XFEL
Beamline	SPB/SFX
Temperature [K]	293
Detector technology	PIXEL
Collection date	2018-06-14
Detector	AGIPD
Wavelength(s)	1.66
Spacegroup name	P 43 21 2
Unit cell lengths	79.850, 79.850, 38.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.710 - 2.200
R-factor	0.2005
Rwork	0.196
R-free	0.23960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6gf0
RMSD bond length	0.002
RMSD bond angle	0.619
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.800	2.300
High resolution limit [Å]	2.200	2.200
Number of reflections	6717
<I/σ(I)>	12
Completeness [%]	100.0	100
Redundancy	1160	690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293	20% NaCl, 6% PEG 6000