6H0C
Flv1 flavodiiron core from Synechocystis sp. PCC6803
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.878, 87.755, 70.665 |
| Unit cell angles | 90.00, 100.94, 90.00 |
Refinement procedure
| Resolution | 57.225 - 1.592 |
| R-factor | 0.148 |
| Rwork | 0.148 |
| R-free | 0.14800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.923 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.230 | 1.700 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmeas | 0.039 | |
| Rpim | 0.022 | |
| Number of reflections | 58995 | |
| <I/σ(I)> | 15.1 | |
| Completeness [%] | 95.7 | |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293.15 | 1 ul of protein at 15 mg/ml with 1 ul of 0.2 M ammonium acetate, 0.1 M tri-sodium citrate pH 5.6, 23-25% (v/v) PEG 4000. |
