6GZT
Structure of Chlamydia trachomatis effector protein ChlaDUB1 bound to Coenzyme A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-28 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 132.635, 132.635, 132.635 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.894 - 2.100 |
| R-factor | 0.1838 |
| Rwork | 0.182 |
| R-free | 0.21580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hag |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.884 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.320 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.085 | 0.653 |
| Number of reflections | 22779 | |
| <I/σ(I)> | 10.3 | 2.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.1 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M HEPES (pH 7.2), 20% PEG 8000 |
