6GZS
Structure of Chlamydia trachomatis effector protein ChlaDUB1 bound to ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-27 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.798, 55.768, 58.574 |
| Unit cell angles | 90.00, 93.57, 90.00 |
Refinement procedure
| Resolution | 26.847 - 1.900 |
| R-factor | 0.1828 |
| Rwork | 0.181 |
| R-free | 0.21320 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.371 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.850 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.043 | 0.476 |
| Number of reflections | 27450 | |
| <I/σ(I)> | 12.4 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M MES (pH 6.0), 20% PEG 6000 |
