6GZP

Llama nanobody PorM_02 structure determined at room temperature by in-situ diffraction in ChipX microfluidic device

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	293.15
Detector technology	PIXEL
Collection date	2017-07-18
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.826561272144
Spacegroup name	P 43 21 2
Unit cell lengths	66.680, 66.680, 91.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.943 - 2.100
R-factor	0.1727
Rwork	0.169
R-free	0.21080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lmw
RMSD bond length	0.008
RMSD bond angle	0.897
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.943	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.064	0.731
Rmeas	0.075	0.847
Rpim	0.037	0.421
Number of reflections	12281
<I/σ(I)>	1.8
Completeness [%]	97.1
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROFLUIDIC		293.15	Tri-sodium Citrate PEG 3000. ChipX microfluidic device