6GZB
Tandem GerMN domains of the sporulation protein GerM from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 273 |
| Detector technology | PIXEL |
| Collection date | 2017-07-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.07252 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 90.360, 103.940, 162.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.460 - 2.100 |
| R-factor | 0.2049 |
| Rwork | 0.204 |
| R-free | 0.23621 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gz8 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.740 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.220 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmeas | 0.065 | 0.590 |
| Number of reflections | 89726 | 13589 |
| <I/σ(I)> | 17.83 | 3.14 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 6 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293.15 | 100 mM Hepes pH 7.2 2.6 M NH4SO4 |
