6GZ2
Crystal Structure of the LeuO Effector Binding Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-25 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 64.990, 88.770, 97.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.020 - 1.940 |
| R-factor | 0.2106 |
| Rwork | 0.209 |
| R-free | 0.23320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.565 |
| Data reduction software | DIALS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3211)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.028 | 1.970 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.082 | 1.670 |
| Rmeas | 0.086 | 1.747 |
| Rpim | 0.024 | 0.505 |
| Number of reflections | 21191 | 1039 |
| <I/σ(I)> | 19.7 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 12.7 | 11.7 |
| CC(1/2) | 1.000 | 0.770 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 1.4 M Sodium malonate pH 7.0, 0.1 M Bis-Tris propane pH 7.0 |
