6GZ0
Crystal Structure of the LeuO Effector Binding Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-06 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.97942 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 63.620, 61.220, 56.540 |
| Unit cell angles | 90.00, 106.24, 90.00 |
Refinement procedure
| Resolution | 54.284 - 1.520 |
| R-factor | 0.1682 |
| Rwork | 0.167 |
| R-free | 0.19320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3oxn |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.053 |
| Data reduction software | DIALS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14rc2_3191: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.284 | 1.550 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.051 | 0.507 |
| Rmeas | 0.006 | 0.582 |
| Rpim | 0.029 | 0.281 |
| Number of reflections | 31127 | 1484 |
| <I/σ(I)> | 10.3 | 0.9 |
| Completeness [%] | 97.0 | 93.98 |
| Redundancy | 4.1 | 4.08 |
| CC(1/2) | 0.998 | 0.813 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.2 M Lithium sulfate, 0.1 M Bis-Tris pH 5.5, 25% polyethylene glycol 3350 |
