6GYW
Crystal structure of DacA from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.799, 68.190, 76.796 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.990 - 1.700 |
| R-factor | 0.1749 |
| Rwork | 0.173 |
| R-free | 0.20040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rv7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.962 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.190 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.078 | 0.627 |
| Number of reflections | 32376 | 1659 |
| <I/σ(I)> | 13.9 | 2 |
| Completeness [%] | 99.9 | 99 |
| Redundancy | 12.2 | 10 |
| CC(1/2) | 0.999 | 0.968 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M LiCl, 0.1M Na-Cacodylate pH 6.5, 30% Peg400 |
