6GYH
Crystal structure of the light-driven proton pump Coccomyxa subellipsoidea Rhodopsin CsR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-04 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.8729 |
| Spacegroup name | H 3 |
| Unit cell lengths | 78.078, 78.078, 143.951 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.910 - 2.000 |
| R-factor | 0.19398 |
| Rwork | 0.192 |
| R-free | 0.22493 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3am6 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.973 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.910 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rpim | 0.066 | 0.901 |
| Number of reflections | 22100 | |
| <I/σ(I)> | 6.8 | 0.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.4 | 4.6 |
| CC(1/2) | 0.997 | 0.357 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 1-(9Z-octadecenoyl)-rac-glycerol, 10 % cholesterol, 42 % v/v polyethylene glycol 400; 100 mM MES; pH 6.5, 150 mM sodium acetate |
