6GY8

Crystal structure of XaxA from Xenorhabdus nematophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.8233
Spacegroup name	P 21 21 21
Unit cell lengths	67.270, 90.830, 153.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.415 - 2.500
R-factor	0.2406
Rwork	0.238
R-free	0.28560
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.462
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	HKL2Map
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.560
High resolution limit [Å]	2.500	2.500
Number of reflections	62589
<I/σ(I)>	18.47
Completeness [%]	99.9
Redundancy	15.93

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293.15	0.2 M sodium chloride, 0.1 M phosphate citrate pH 4.2, 10 % PEG 3000
1	VAPOR DIFFUSION, SITTING DROP	4.2	293.15	0.2 M sodium chloride, 0.1 M phosphate citrate pH 4.2, 10 % PEG 3000