6GXZ
Crystal structure of the human RPAP3(TPR2)-PIH1D1(CS) complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.96770 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 135.980, 42.440, 112.710 |
| Unit cell angles | 90.00, 98.25, 90.00 |
Refinement procedure
| Resolution | 67.287 - 2.965 |
| R-factor | 0.2261 |
| Rwork | 0.223 |
| R-free | 0.28370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fdp |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.580 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.287 | 3.016 |
| High resolution limit [Å] | 2.965 | 2.965 |
| Number of reflections | 13488 | 1345 |
| <I/σ(I)> | 11.6 | |
| Completeness [%] | 99.0 | 99.26 |
| Redundancy | 7 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG-3350 25%, CaCl2 50mM, Tris-HCL 100mM pH 7.7 |
