6GXS
Crystal structure of CV39L lectin from Chromobacterium violaceum at 1.8 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-31 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.967 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.709, 123.781, 180.935 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 73.040 - 1.800 |
R-factor | 0.15458 |
Rwork | 0.153 |
R-free | 0.18852 |
Structure solution method | SAD |
RMSD bond length | 0.023 |
RMSD bond angle | 2.014 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.930 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.102 | 0.363 |
Number of reflections | 137285 | 19822 |
<I/σ(I)> | 14.7 | 4.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.5 | 7.5 |
CC(1/2) | 0.999 | 0.953 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 290 | 0.1 M sodium acetate, 25% PEG 4000, 0.2M ammonium sulphate, pH 4.6 |