6GWV

Molybdenum storage protein without polymolybdate clusters and ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-10
Detector	PSI PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	116.710, 147.300, 192.320
Unit cell angles	90.00, 107.62, 90.00

Refinement procedure

Resolution	47.794 - 2.800
R-factor	0.2413
Rwork	0.233
R-free	0.25930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.084
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	148308
<I/σ(I)>	8.4
Completeness [%]	92.2
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293.15	0.1 M HEPES, pH 7.5, 0.4 M ammonium sulfate, 20% PEG400, 10% isopropanol