6GWK

The crystal structure of Hfq from Caulobacter crescentus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-27
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97625
Spacegroup name	P 43
Unit cell lengths	97.322, 97.322, 203.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	87.810 - 2.150
R-factor	0.2797
Rwork	0.278
R-free	0.30510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pno
RMSD bond length	0.003
RMSD bond angle	0.520
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_3112)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	87.810	2.227
High resolution limit [Å]	2.150	2.150
Rmerge	0.123	0.628
Rmeas	0.155	0.823
Rpim	0.092	0.525
Number of reflections	100169	10091
<I/σ(I)>	9.44	0.46
Completeness [%]	97.4	99.06
Redundancy	2	1.9
CC(1/2)	0.990	0.618

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.2M sodium thiocyanate, 17 % PEG 3350