6GWI
The crystal structure of Halomonas elongata amino-transferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-11 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.344, 61.875, 67.475 |
| Unit cell angles | 83.45, 82.58, 72.52 |
Refinement procedure
| Resolution | 46.915 - 2.000 |
| R-factor | 0.1622 |
| Rwork | 0.160 |
| R-free | 0.21000 |
| Data reduction software | X-Area |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.920 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.104 | 0.429 |
| Rmeas | 0.147 | |
| Rpim | 0.104 | 0.429 |
| Number of reflections | 54196 | 3885 |
| <I/σ(I)> | 8.5 | 2.4 |
| Completeness [%] | 93.4 | 90.7 |
| Redundancy | 1.7 | 1.6 |
| CC(1/2) | 0.997 | 0.831 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.9 | 293 | Hampton Research PEG/ION screen condition 25 (20% PEG3350, 0.2M magnesium acetate, pH 7.9). |
