6GWC

Tubulin:iE5 alphaRep complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97857
Spacegroup name	P 32 2 1
Unit cell lengths	102.346, 102.346, 216.207
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.250 - 2.600
R-factor	0.176
Rwork	0.173
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4drx 3ltj
RMSD bond length	0.010
RMSD bond angle	1.160
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.250	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	41238
<I/σ(I)>	14.6
Completeness [%]	99.9
Redundancy	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	293	13% polyethylene glycol 400, 0.1 M Mes-K pH 6.8