6GVR
TAILSPIKE PROTEIN MUTANT E372Q (DELTA N471/S472) OF E. COLI BACTERIOPHAGE HK620 IN COMPLEX WITH PENTASACCHARIDE
Replaces: 4XQIExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 74.164, 74.164, 174.845 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.710 - 1.850 |
| R-factor | 0.151 |
| Rwork | 0.149 |
| R-free | 0.19670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.972 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.710 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmeas | 0.121 | 0.901 |
| Number of reflections | 48191 | 2820 |
| <I/σ(I)> | 16 | 2.2 |
| Completeness [%] | 99.7 | 95.8 |
| Redundancy | 6.1 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M TRIS-HCL, 3.5 M SODIUM FORMATE pH 8.5 |
