6GV1

Crystal structure of E.coli Multidrug/H+ antiporter MdfA in outward open conformation with bound Fab fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 61 2 2
Unit cell lengths	73.258, 73.258, 927.920
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.048 - 3.400
R-factor	0.2588
Rwork	0.258
R-free	0.28310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zp0 1ibg
RMSD bond length	0.003
RMSD bond angle	0.596
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.048	49.048	3.610
High resolution limit [Å]	3.400	10.010	3.400
Rmerge	0.254	0.036	1.669
Rmeas	0.262	0.038	1.724
Number of reflections	22224	1076	3423
<I/σ(I)>	11.57	51.59	1.59
Completeness [%]	99.9	98.4	99.9
Redundancy	17.3	14.422	16.039
CC(1/2)	1.000	1.000	0.583

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6.4	293	100 mM ADA pH 6.5 100 mM NaCl 100 mM Li2SO4 32-36% PEG 300 8.8 MAG