6GUQ

Crystal structure of GanP, a glucose-galactose binding protein from Geobacillus stearothermophilus, in complex with glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-17
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.968
Spacegroup name	P 31 2 1
Unit cell lengths	70.970, 70.970, 149.190
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.660 - 2.385
R-factor	0.2006
Rwork	0.198
R-free	0.24410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3c6q
RMSD bond length	0.008
RMSD bond angle	0.889
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.440	2.530
High resolution limit [Å]	2.380	2.380
Rmerge	0.111	1.870
Number of reflections	17967	2831
<I/σ(I)>	29.37	2.43
Completeness [%]	99.8	99.2
Redundancy	29.81	2.43
CC(1/2)	1.000	0.944

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M HEPES (pH 7.5 buffer), and 20% w/v PEG 10K. Crystals were soaked with 30 mM of glucose and 15% PEG 10K solution.