6GT9

Crystal structure of GanP, a glucose-galactose binding protein from Geobacillus stearothermophilus, in complex with galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	71.520, 88.890, 88.880
Unit cell angles	90.00, 95.41, 90.00

Refinement procedure

Resolution	58.217 - 1.894
R-factor	0.1926
Rwork	0.192
R-free	0.23910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3c6q
RMSD bond length	0.007
RMSD bond angle	0.881
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.890	1.930
High resolution limit [Å]	1.890	1.890
Rmerge	0.119	0.824
Number of reflections	87725	4369
<I/σ(I)>	12	2.3
Completeness [%]	99.6	97.4
Redundancy	7.3	6.6
CC(1/2)	0.998	0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	293	0.1 M sodium acetate (pH 4 buffer), 26%-28% w/v PEG MME 2K, 0.2 M ammonium sulfate.