6GT2
Reduced copper nitrite reductase from Achromobacter cycloclastes determined by serial femtosecond rotation crystallography
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SACLA BEAMLINE BL2 |
Synchrotron site | SACLA |
Beamline | BL2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-10-05 |
Detector | RAYONIX MX225-HS |
Wavelength(s) | 1.242 |
Spacegroup name | P 21 3 |
Unit cell lengths | 94.610, 94.610, 94.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.680 - 1.600 |
R-factor | 0.16823 |
Rwork | 0.167 |
R-free | 0.19910 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.533 |
Data reduction software | CrystFEL (0.6.3) |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.620 | 1.640 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 37489 | 1858 |
<I/σ(I)> | 5.4 | 2.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 66.1 | 44.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293.15 | 1.2 M ammonium sulphate, 100 mM citrate buffer, pH 5.0 |