6GRU
Crystal structure of human NUDT5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-11 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9159 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.600, 60.140, 80.010 |
| Unit cell angles | 79.92, 81.52, 75.49 |
Refinement procedure
| Resolution | 32.030 - 1.930 |
| R-factor | 0.23298 |
| Rwork | 0.231 |
| R-free | 0.26960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dsd |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.694 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.490 | 1.980 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.035 | |
| Number of reflections | 64137 | |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 97.5 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.15 | 30% PEG4000, 0.2M magnesium chloride, 0.1M tris pH 8.5 |
