6GRK
Structure of the soluble AhlB of the tripartite alpha-pore forming toxin, AHL, from Aeromonas hydrophila.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-11 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 133.470, 79.810, 111.010 |
| Unit cell angles | 90.00, 90.22, 90.00 |
Refinement procedure
| Resolution | 58.410 - 2.330 |
| R-factor | 0.22391 |
| Rwork | 0.222 |
| R-free | 0.25361 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.408 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.340 | 2.390 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Number of reflections | 49043 | |
| <I/σ(I)> | 0.791 | |
| Completeness [%] | 98.1 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M potassium thiocyanate, 0.1 M bis-tris propane pH 6.5, and 20 % PEG3350 |
