6GQS
Structure of human Heat shock protein 90-alpha N-terminal domain (Hsp90-NTD) variant K112R in complex with AMPCP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-19 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97622 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.791, 42.173, 54.572 |
Unit cell angles | 90.00, 116.61, 90.00 |
Refinement procedure
Resolution | 48.790 - 1.430 |
R-factor | 0.16358 |
Rwork | 0.162 |
R-free | 0.18613 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xk2 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.849 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.790 | 1.510 |
High resolution limit [Å] | 1.430 | 1.430 |
Rmerge | 0.064 | 0.360 |
Rmeas | 0.075 | 0.419 |
Rpim | 0.038 | 0.212 |
Number of reflections | 40642 | 5906 |
<I/σ(I)> | 11.4 | 3.3 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 3.8 | 3.7 |
CC(1/2) | 0.995 | 0.855 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | Precipitant: 25 % wt/vol PEG 2000, 200mM MgCl2, 100 mM sodium cacodylate, pH 6.5 Sample: Hsp90a-NTD K112R 20 mg/mL, 10 mM AMPCP, 500 mM NaCl, 20 mM TRIS, pH 7.5 |