6GQH
Structure of GFPmut2 crystallized at pH 8.5 and transferred to pH 6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-10-30 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.20 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.295, 62.183, 69.625 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.379 - 2.400 |
| R-factor | 0.1935 |
| Rwork | 0.191 |
| R-free | 0.23650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q4a |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.149 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.2.14) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.380 | 46.380 | 2.490 |
| High resolution limit [Å] | 2.400 | 8.980 | 2.400 |
| Rmerge | 0.105 | 0.049 | 0.697 |
| Rmeas | 0.115 | 0.054 | 0.757 |
| Rpim | 0.045 | 0.022 | 0.291 |
| Total number of observations | 57671 | ||
| Number of reflections | 9172 | 213 | 935 |
| <I/σ(I)> | 11.9 | ||
| Completeness [%] | 100.0 | 99.8 | 100 |
| Redundancy | 6.3 | 5.5 | 6.6 |
| CC(1/2) | 0.997 | 0.998 | 0.877 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 277 | 0.2 M CaCl2, 19% PEG 4000 |
