6GQA
Cell division regulator S. pneumoniae GpsB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER METALJET |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-31 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.34 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.119, 54.454, 61.637 |
| Unit cell angles | 90.00, 106.13, 90.00 |
Refinement procedure
| Resolution | 26.010 - 1.900 |
| R-factor | 0.1911 |
| Rwork | 0.188 |
| R-free | 0.24930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ug1 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.399 |
| Data reduction software | SAINT |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.220 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rpim | 0.041 | 0.311 |
| Number of reflections | 19776 | |
| <I/σ(I)> | 13.9 | 4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 25.2 | 20.7 |
| CC(1/2) | 0.998 | 0.780 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Tris.HCl pH 8.5, 0.2M magnesium chloride, 20% PEG 8000 |
