6GPW
Structure of human Heat shock protein 90-alpha N-terminal domain (Hsp90-NTD)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-23 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97950 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 65.040, 88.890, 100.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.450 - 1.600 |
| R-factor | 0.16841 |
| Rwork | 0.167 |
| R-free | 0.19947 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uyl |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.937 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.450 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.057 | 0.390 |
| Rmeas | 0.060 | 0.417 |
| Rpim | 0.021 | 0.143 |
| Number of reflections | 38609 | 5575 |
| <I/σ(I)> | 18.4 | 4.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.3 | 8.1 |
| CC(1/2) | 0.999 | 0.930 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 25 % wt/vol PEG 2000, 200mM MgCl2, 100 mM sodium cacodylate, pH 6.5 |
