6GPV
Crystal structure of blue-light irradiated miniSOG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-23 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 40.500, 40.500, 133.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.760 - 2.000 |
| R-factor | 0.1825 |
| Rwork | 0.180 |
| R-free | 0.24020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4eep |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.554 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmeas | 0.176 | 1.060 |
| Number of reflections | 8116 | 1064 |
| <I/σ(I)> | 12.7 | 2.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 8.2 | 8.7 |
| CC(1/2) | 0.997 | 0.735 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 100 mM Tris-Hcl pH 8.0, 20 mM MgCl2, 28% PEG 4000, 0 or 15 mM CoCl2 |
