6GPU
Crystal structure of miniSOG at 1.17A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-23 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 40.020, 40.020, 134.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.350 - 1.170 |
| R-factor | 0.13414 |
| Rwork | 0.133 |
| R-free | 0.16400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4eep |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.700 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 1.200 |
| High resolution limit [Å] | 1.170 | 1.170 |
| Rmeas | 0.062 | 1.205 |
| Number of reflections | 37913 | 2747 |
| <I/σ(I)> | 22.1 | 2 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 8.2 | 8 |
| CC(1/2) | 1.000 | 0.704 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 100 mM Tris-Hcl pH 8.0, 20 mM MgCl2, 28% PEG 4000, 0 or 15 mM CoCl2 |
