6GPA
Beta-1,4-galactanase from Bacteroides thetaiotaomicron with galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.976200 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.544, 45.632, 138.760 |
| Unit cell angles | 90.00, 99.80, 90.00 |
Refinement procedure
| Resolution | 46.840 - 1.790 |
| R-factor | 0.1728 |
| Rwork | 0.170 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gp5 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.457 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.3.11) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.850 | 46.850 | 1.830 |
| High resolution limit [Å] | 1.790 | 8.960 | 1.790 |
| Rmerge | 0.110 | 0.051 | 0.403 |
| Rmeas | 0.140 | 0.065 | 0.522 |
| Rpim | 0.085 | 0.039 | 0.328 |
| Total number of observations | 138347 | ||
| Number of reflections | 53053 | 442 | 2687 |
| <I/σ(I)> | 8.6 | ||
| Completeness [%] | 96.1 | 93.2 | 80.5 |
| Redundancy | 2.6 | 2.7 | 2.4 |
| CC(1/2) | 0.974 | 0.990 | 0.514 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 20-23% PEG6000, 200 mM NH4Cl, MES pH 6.0 |
