6GP6
MamM CTD - Copper form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-23 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.96771 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 28.930, 73.750, 89.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.705 - 2.146 |
| R-factor | 0.2231 |
| Rwork | 0.220 |
| R-free | 0.27510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w5x |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.076 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.710 | 44.710 | 2.200 |
| High resolution limit [Å] | 2.140 | 9.080 | 2.140 |
| Rmerge | 0.106 | 0.049 | 1.863 |
| Rmeas | 0.118 | 0.055 | 2.044 |
| Rpim | 0.049 | 0.024 | 0.828 |
| Total number of observations | 64227 | 841 | 5042 |
| Number of reflections | 11127 | 184 | 864 |
| <I/σ(I)> | 8.5 | 22.1 | 1 |
| Completeness [%] | 99.6 | 98.1 | 96.9 |
| Redundancy | 5.8 | 4.6 | 5.8 |
| CC(1/2) | 0.996 | 0.998 | 0.436 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M NaCl, 0.1M Tris pH=8.7, 25% PEG 3350, 1.7 mM CuSO4 |
