6GP2
Ribonucleotide Reductase class Ie R2 from Mesoplasma florum, DOPA-active form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-09 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.96859 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 176.162, 53.744, 79.284 |
Unit cell angles | 90.00, 108.58, 90.00 |
Refinement procedure
Resolution | 42.730 - 1.480 |
Rwork | 0.154 |
R-free | 0.18500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dhz |
RMSD bond length | 0.006 |
RMSD bond angle | 0.800 |
Data reduction software | XDS (VERSION Jan 26, 2018) |
Data scaling software | XDS (VERSION Jan 26, 2018) |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: 000)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.730 | 1.532 |
High resolution limit [Å] | 1.479 | 1.479 |
Rmerge | 0.066 | 1.403 |
Rmeas | 0.072 | 1.530 |
Rpim | 0.028 | 0.602 |
Number of reflections | 117391 | 11678 |
<I/σ(I)> | 12.67 | 1.17 |
Completeness [%] | 99.8 | 99.65 |
Redundancy | 6.6 | 6.4 |
CC(1/2) | 0.999 | 0.613 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 100-200 mM calcium acetate, 100 mM ammonium sulfate, 15-20% PEG 3350 |