6GP2
Ribonucleotide Reductase class Ie R2 from Mesoplasma florum, DOPA-active form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.96859 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 176.162, 53.744, 79.284 |
| Unit cell angles | 90.00, 108.58, 90.00 |
Refinement procedure
| Resolution | 42.730 - 1.480 |
| Rwork | 0.154 |
| R-free | 0.18500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dhz |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.800 |
| Data reduction software | XDS (VERSION Jan 26, 2018) |
| Data scaling software | XDS (VERSION Jan 26, 2018) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: 000)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.730 | 1.532 |
| High resolution limit [Å] | 1.479 | 1.479 |
| Rmerge | 0.066 | 1.403 |
| Rmeas | 0.072 | 1.530 |
| Rpim | 0.028 | 0.602 |
| Number of reflections | 117391 | 11678 |
| <I/σ(I)> | 12.67 | 1.17 |
| Completeness [%] | 99.8 | 99.65 |
| Redundancy | 6.6 | 6.4 |
| CC(1/2) | 0.999 | 0.613 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 100-200 mM calcium acetate, 100 mM ammonium sulfate, 15-20% PEG 3350 |
