6GOX
SecA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 163.140, 106.830, 75.980 |
| Unit cell angles | 90.00, 102.81, 90.00 |
Refinement procedure
| Resolution | 88.690 - 3.500 |
| R-factor | 0.2344 |
| Rwork | 0.232 |
| R-free | 0.29270 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.461 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.690 | 3.710 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 31379 | |
| <I/σ(I)> | 9.44 | |
| Completeness [%] | 99.4 | |
| Redundancy | 3.45 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 18% PEG 3350, 0.054 BIS-TRIS propane, pH6.4, 0.036M citrate, plus additives |
