6GOL

Three dimensional structure of a novel influenza hemagglutinin tri-stalk protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-3
Synchrotron site	MAX II
Beamline	I911-3
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-04
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.00000
Spacegroup name	P 63
Unit cell lengths	41.800, 41.800, 90.120
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.120 - 1.700
R-factor	0.17959
Rwork	0.178
R-free	0.21914
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1htm
RMSD bond length	0.024
RMSD bond angle	2.009
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.120	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.063	0.664
Number of reflections	9803	1439
<I/σ(I)>	10	1.8
Completeness [%]	99.7	100
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	294	2.0 M ammonium sulfate and 0.1 M Bis-Tris, pH 5.5, protein 10 mg/mL