6GO9

Structure of GFPmut2 crystallized at pH 6 and transferred to pH 7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-08
Detector	ADSC QUANTUM 315
Wavelength(s)	1.2
Spacegroup name	P 21 21 21
Unit cell lengths	32.515, 60.027, 112.262
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.999 - 1.672
R-factor	0.1627
Rwork	0.161
R-free	0.19310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q4a
RMSD bond length	0.006
RMSD bond angle	1.114
Data reduction software	XDS
Data scaling software	Aimless (0.2.14)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.000	41.000	1.700
High resolution limit [Å]	1.670	9.010	1.670
Rmerge	0.098	0.048	0.494
Rmeas	0.106	0.053	0.550
Rpim	0.040	0.021	0.234
Total number of observations	175812
Number of reflections	26154	214	1207
<I/σ(I)>	12.5
Completeness [%]	99.5	98.6	90
Redundancy	6.7	5.3	4.9
CC(1/2)	0.998	0.998	0.812

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	277	45% MPD