6GO9
Structure of GFPmut2 crystallized at pH 6 and transferred to pH 7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.2 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.515, 60.027, 112.262 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.999 - 1.672 |
| R-factor | 0.1627 |
| Rwork | 0.161 |
| R-free | 0.19310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q4a |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.114 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.2.14) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 41.000 | 41.000 | 1.700 |
| High resolution limit [Å] | 1.670 | 9.010 | 1.670 |
| Rmerge | 0.098 | 0.048 | 0.494 |
| Rmeas | 0.106 | 0.053 | 0.550 |
| Rpim | 0.040 | 0.021 | 0.234 |
| Total number of observations | 175812 | ||
| Number of reflections | 26154 | 214 | 1207 |
| <I/σ(I)> | 12.5 | ||
| Completeness [%] | 99.5 | 98.6 | 90 |
| Redundancy | 6.7 | 5.3 | 4.9 |
| CC(1/2) | 0.998 | 0.998 | 0.812 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 277 | 45% MPD |
