6GO8

Structure of GFPmut2 crystallized at pH 6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	CCD
Collection date	2008-05-26
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	32.504, 60.009, 112.497
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.143 - 1.648
R-factor	0.1564
Rwork	0.155
R-free	0.17400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q4a
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	XDS
Data scaling software	Aimless (0.2.14)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.040	41.040	1.680
High resolution limit [Å]	1.648	9.030	1.648
Rmerge	0.059	0.039	0.217
Rmeas	0.067	0.046	0.266
Rpim	0.031	0.024	0.149
Number of reflections	25496	168	1183
<I/σ(I)>	15.7
Completeness [%]	93.5	80.9	89.3
Redundancy	3.8	3.5	2.5
CC(1/2)	0.997	0.998	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	277	45% MPD