6GNX
Crystal structure of the MAJIN-TERB2 heterotetrameric complex - selenomethionine derivative
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-15 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9159 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 59.880, 59.880, 159.930 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.329 - 2.900 |
R-factor | 0.2589 |
Rwork | 0.254 |
R-free | 0.30390 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.539 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((dev_3126: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.330 | 3.080 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmeas | 0.136 | 3.015 |
Rpim | 0.024 | 0.553 |
Number of reflections | 7915 | 1250 |
<I/σ(I)> | 28.2 | 2.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 56.6 | 55.1 |
CC(1/2) | 1.000 | 0.885 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.12M 1,6-Hexanediol; 0.12M 1-Butanol; 0.12M 1,2-Propanediol (racemic); 0.12M 2-Propanol; 0.12M 1,4-Butanediol; 0.12M 1,3-Propanediol, 55.5 mM MES pH 3.11, 44.5 mM imidazole pH 10.23; 12.5% w/v PEG 1000; 12.5% w/v PEG 3350; 12.5% v/v MPD |