6GNF
Granule Bound Starch Synthase from Cyanobacterium sp. CLg1 bound to acarbose and ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.953 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 194.260, 132.650, 123.690 |
| Unit cell angles | 90.00, 126.30, 90.00 |
Refinement procedure
| Resolution | 48.560 - 2.200 |
| R-factor | 0.20523 |
| Rwork | 0.205 |
| R-free | 0.22377 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vue |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.151 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 200.000 | 2.400 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmeas | 0.083 | 1.470 |
| Number of reflections | 121565 | 23165 |
| <I/σ(I)> | 14.03 | |
| Completeness [%] | 94.7 | 78.8 |
| Redundancy | 6.2 | 4.1 |
| CC(1/2) | 0.999 | 0.480 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 288 | 2M (NH4)2SO4, 2% PEG400 and 150 mM Hepes buffer at pH 7.5, acarbose, ADP |
