6GN8
Exoenzyme S from Pseudomonas aeruginosa in complex with human 14-3-3 protein beta, trimeric crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-23 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97624 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 159.909, 59.401, 120.333 |
Unit cell angles | 90.00, 125.79, 90.00 |
Refinement procedure
Resolution | 97.610 - 2.340 |
R-factor | 0.202 |
Rwork | 0.200 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6gn0 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.050 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 97.610 | 2.380 |
High resolution limit [Å] | 2.340 | 2.340 |
Rmerge | 0.092 | 1.389 |
Rmeas | 0.100 | 1.541 |
Rpim | 0.039 | 0.657 |
Number of reflections | 39047 | 1928 |
<I/σ(I)> | 10.3 | 1.2 |
Completeness [%] | 99.9 | 99.1 |
Redundancy | 6.5 | 5.5 |
CC(1/2) | 0.999 | 0.621 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 40% MPD, 8% PEG 8000, 0.1M Sodium Cacodylate |